The method uses adjustable nanopore technology—which allows the passage of ionic current and particles to be managed by modifying the pore size—in conjunction with resistive pulse-sensing, which detects current flow via an aperture. A brief alteration in the ionic current flow, referred to as a blockade event, is observed when particles pass through a pore one at a time. The blockade magnitude is the amplitude of this change. After calibrating with a known standard, precise exosome concentration measurement can be obtained because blockade magnitude is proportionate to particle size.
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